Journal: Frontiers in Microbiology

Article Title: Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry

doi: 10.3389/fmicb.2019.00856

Figure Lengend Snippet: Detection of authentic tibroviruses in nonhuman cell lines.

Article Snippet: Vero cells and embryonic grivet kidney MA104 cells (ATCC, #CCL-2378) were grown at 37°C in a humidified 5% CO 2 atmosphere in DMEM.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry

doi: 10.3389/fmicb.2019.00856

Figure Lengend Snippet: Tibrovirus glycoproteins mediate virion entry into a broad range of animal cell types. Same experiment as in , using different cell types exposed to rVSIV–VSIV G control and rVSIVs expressing diverse tibrovirus glycoproteins (G) (MOI = 3). (A) Bat (PESU-B5L, Ro5T, Ro6E, EidNi/41.3, EpoNi/22.1, RoNi/7.1, RoNi/7.2, HypNi/1.1, HypLu/45.1, Tb1 Lu, MyDauLu/47.1), nonhuman primate (Vero, MA104, RPGor53, S008397, RP00226), hispid cotton rat CRL, and boa constrictor JK cell lines. (B) Asian tiger mosquito C6/36 cells. The percentage of eGFP-expressing cell lines was measured by high-content imaging at 24 h post-exposure (bat, nonhuman primate, hispid cotton rat, and boa constrictor cell lines) or at 24, 48, 72, and 96 h post-exposure (Asian tiger mosquito cells). All experiments were performed in triplicate; error bars show standard deviations. BHV, Beatrice Hill virus; BASV, Bas-Congo virus; BAV, Bivens Arm virus; CPV, Coastal Plains virus; eGFP, enhanced green fluorescent protein; EKV-1, Ekpoma virus 1; EKV-2, Ekpoma virus 2; MOI, multiplicity of infection; SWBV, Sweetwater Branch virus; TIBV, Tibrogargan virus; rVSIV, recombinant vesicular stomatitis Indiana virus.

Article Snippet: Vero cells and embryonic grivet kidney MA104 cells (ATCC, #CCL-2378) were grown at 37°C in a humidified 5% CO 2 atmosphere in DMEM.

Techniques: Control, Expressing, Imaging, Virus, Infection, Recombinant